RPMI 8226 is a B lymphocyte that was isolated from the peripheral blood of a 61-year-old, male with plasmacytoma. The cell can be used in assay development and immunology research. The cell line can be used in testing and calibration in ISO 17025-accredited laboratories, to challenge assay performance, validate or compare test methods, and establish sensitivity, linearity, and specificity during assay validation or implementation.